lsl enascas12a mice Search Results


lsl enascas12a mice  (Jackson Laboratory)
86
Jackson Laboratory lsl enascas12a mice
Lsl Enascas12a Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lsl+enascas12a+mice/jax+stock/pmc12360953-367-11-16
Average 86 stars, based on 1 article reviews
lsl enascas12a mice - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
cas12a mice  (Jackson Laboratory)
86
Jackson Laboratory cas12a mice
a , Schematic showing the breeding strategy of LSL-enAsCas12a-HF1;dCas9-SPH mice. Created in BioRender. Chen, S. (2025) https://BioRender.com/h16k998 . b , Schematic demonstrating the mechanism of the simultaneous DAKO system. A dCas9-sgRNA was concatenated with <t>Cas12a</t> DR and crRNA. Upon delivery into the same cell, the guide chimera was then processed and cleaved into mature dCas9-sgRNA and Cas12a-crRNA by Cas12a protein. The mature dCas9-sgRNA and Cas12a-crRNA then assembled with corresponding protein to mediate gene activation and gene knockout, respectively. RNP, ribonucleoprotein. Created in BioRender. Chen, S. (2025) https://BioRender.com/t91r170 . c , Schematic illustrating the retroviral construct and the workflow of ex vivo of DAKO validation in primary BMDCs. Human U6 promoter (hU6) drives the expression of a Cas9 activating guide for Itgb4 (Itgb4-sgRNA) concatenated with a Cas12a DR and crRNA targeting CD24 (crCD24). A Cre expression cassette was used to induce enAsCas12a-HF1 expression. BM, bone marrow. Created in BioRender. Chen, S. (2025) https://BioRender.com/d07o322 . d , Flow cytometry analysis on LSL-enAsCas12a BMDC Itgb4-CD24 DAKO experiment. Representative contour plots of mScarlet + BMDCs showing the Itgb4 expression in relation to the CD24 expression. Anti-ITGB4-APC and anti-CD24-BV421 were used in the flow staining. DAKO-crRNA containing both crItgb4 and crCD24 was compared with single crRNA (Itgb4-sgRNA and crCD24) and vector control. e , Quantification of CD24 − , ITGB4 + and CD24 − ;Itgb4 + percentage with respect to mScarlet + BMDCs. One-way ANOVA with Tukey’s multiple comparisons test was used to assess significance. For bar plot, data are shown as mean ± s.e.m. Exact P values are labelled. For all groups, N = 3 biological replicates.
Cas12a Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lsl+enascas12a+mice/cas12a+mice/pmc12360953-637-1-6
Average 86 stars, based on 1 article reviews
cas12a mice - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


a , Schematic showing the breeding strategy of LSL-enAsCas12a-HF1;dCas9-SPH mice. Created in BioRender. Chen, S. (2025) https://BioRender.com/h16k998 . b , Schematic demonstrating the mechanism of the simultaneous DAKO system. A dCas9-sgRNA was concatenated with Cas12a DR and crRNA. Upon delivery into the same cell, the guide chimera was then processed and cleaved into mature dCas9-sgRNA and Cas12a-crRNA by Cas12a protein. The mature dCas9-sgRNA and Cas12a-crRNA then assembled with corresponding protein to mediate gene activation and gene knockout, respectively. RNP, ribonucleoprotein. Created in BioRender. Chen, S. (2025) https://BioRender.com/t91r170 . c , Schematic illustrating the retroviral construct and the workflow of ex vivo of DAKO validation in primary BMDCs. Human U6 promoter (hU6) drives the expression of a Cas9 activating guide for Itgb4 (Itgb4-sgRNA) concatenated with a Cas12a DR and crRNA targeting CD24 (crCD24). A Cre expression cassette was used to induce enAsCas12a-HF1 expression. BM, bone marrow. Created in BioRender. Chen, S. (2025) https://BioRender.com/d07o322 . d , Flow cytometry analysis on LSL-enAsCas12a BMDC Itgb4-CD24 DAKO experiment. Representative contour plots of mScarlet + BMDCs showing the Itgb4 expression in relation to the CD24 expression. Anti-ITGB4-APC and anti-CD24-BV421 were used in the flow staining. DAKO-crRNA containing both crItgb4 and crCD24 was compared with single crRNA (Itgb4-sgRNA and crCD24) and vector control. e , Quantification of CD24 − , ITGB4 + and CD24 − ;Itgb4 + percentage with respect to mScarlet + BMDCs. One-way ANOVA with Tukey’s multiple comparisons test was used to assess significance. For bar plot, data are shown as mean ± s.e.m. Exact P values are labelled. For all groups, N = 3 biological replicates.

Journal: Nature Biomedical Engineering

Article Title: Cas12a-knock-in mice for multiplexed genome editing, disease modelling and immune-cell engineering

doi: 10.1038/s41551-025-01371-2

Figure Lengend Snippet: a , Schematic showing the breeding strategy of LSL-enAsCas12a-HF1;dCas9-SPH mice. Created in BioRender. Chen, S. (2025) https://BioRender.com/h16k998 . b , Schematic demonstrating the mechanism of the simultaneous DAKO system. A dCas9-sgRNA was concatenated with Cas12a DR and crRNA. Upon delivery into the same cell, the guide chimera was then processed and cleaved into mature dCas9-sgRNA and Cas12a-crRNA by Cas12a protein. The mature dCas9-sgRNA and Cas12a-crRNA then assembled with corresponding protein to mediate gene activation and gene knockout, respectively. RNP, ribonucleoprotein. Created in BioRender. Chen, S. (2025) https://BioRender.com/t91r170 . c , Schematic illustrating the retroviral construct and the workflow of ex vivo of DAKO validation in primary BMDCs. Human U6 promoter (hU6) drives the expression of a Cas9 activating guide for Itgb4 (Itgb4-sgRNA) concatenated with a Cas12a DR and crRNA targeting CD24 (crCD24). A Cre expression cassette was used to induce enAsCas12a-HF1 expression. BM, bone marrow. Created in BioRender. Chen, S. (2025) https://BioRender.com/d07o322 . d , Flow cytometry analysis on LSL-enAsCas12a BMDC Itgb4-CD24 DAKO experiment. Representative contour plots of mScarlet + BMDCs showing the Itgb4 expression in relation to the CD24 expression. Anti-ITGB4-APC and anti-CD24-BV421 were used in the flow staining. DAKO-crRNA containing both crItgb4 and crCD24 was compared with single crRNA (Itgb4-sgRNA and crCD24) and vector control. e , Quantification of CD24 − , ITGB4 + and CD24 − ;Itgb4 + percentage with respect to mScarlet + BMDCs. One-way ANOVA with Tukey’s multiple comparisons test was used to assess significance. For bar plot, data are shown as mean ± s.e.m. Exact P values are labelled. For all groups, N = 3 biological replicates.

Article Snippet: The Cas12a mice are available from The Jackson Laboratory (JAX), with JAX Stock numbers LSL-enAsCas12a-HF1 (039526), enAsCas12a-HF1 (039527), LSL-LbCas12a (039528) and LbCas12a (039529). are provided with this paper.

Techniques: Activation Assay, Gene Knockout, Retroviral, Construct, Ex Vivo, Biomarker Discovery, Expressing, Flow Cytometry, Staining, Plasmid Preparation, Control